ELI5: Explain Like I'm 5

Capillary electrophoresis

Okay kiddo, so if you imagine that all the stuff in a liquid, like water or juice, is made up of tiny building blocks called molecules. These molecules are so small that you can't see them with your eyes, but scientists have special tools that can help them separate and study the different types of molecules.

One of these tools is called capillary electrophoresis. It's like a magic machine that helps scientists sort and measure the molecules in a liquid sample. Here's how it works:

First, the scientist takes a small amount of the liquid they want to study and puts it into a tiny glass tube called a capillary. This tube is really, really thin - like as thin as the tip of a needle - and it has a special coating on the inside that helps the molecules move through it.

Next, the scientist attaches one end of the capillary to a machine that uses electricity to create a special type of force called an electric field. This force travels through the capillary and pulls the molecules in the liquid along with it.

But here's the cool part: different types of molecules have different properties, like size, shape, and charge. This means that when they move through the capillary under the electric field, they end up in different places! Bigger molecules move more slowly, while smaller ones move faster. And molecules with different charges get pulled towards the positive or negative end of the capillary.

So, as the molecules travel through the capillary, they start to separate out from each other and form different "bands" or zones. The scientist can then use a detector to measure the amount of each type of molecule in each zone, and create a picture or graph showing what the sample is made up of.

Capillary electrophoresis is a really helpful tool, because it can be used to study all sorts of things, like DNA, proteins, drugs, and chemicals in food or medicine. Scientists can use it to figure out what's in a sample, how much of each thing is there, and even how the molecules interact with each other. And the best part is, it's all invisible to the naked eye, so it's like a superpower that helps scientists see things they couldn't see before!