ELI5: Explain Like I'm 5

Enzyme assay

Okay, kiddo! So, enzymes are like tiny little helpers inside our bodies that help us do things like digest our food and breathe. And just like how we need to see how well we're doing on a test, scientists need to measure how well these enzymes are working too! That's where enzyme assays come in.

An enzyme assay is kinda like a test that measures how well an enzyme is doing its job. Scientists put a little bit of the enzyme in a special container and add some other chemicals called substrates. These substrates are kinda like puzzles that the enzyme needs to solve.

As the enzyme works on these substrates, it changes them into something else. Scientists can measure this change to see how well the enzyme is working. It's like seeing how many pieces of a puzzle you can solve in a certain amount of time.

Sometimes, scientists need to make sure that their results are accurate, so they might use a control. A control is like a test where they don't add the enzyme, just to make sure any changes they see are actually because of the enzyme, and not something else.

So there you have it, kiddo! Enzyme assays are just tests that measure how well enzymes are working, by seeing how they change other chemicals!
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