Isoelectric focusing
Okay kiddo, let me explain to you what isoelectric focusing means!
You know how we have different types of molecules in our body, right? And some of these molecules have an electric charge?
Isoelectric focusing is a method that scientists use to separate these molecules based on their electric charges. But how do they do this, you may ask?
Well, they start by placing the mixture of these molecules on a special gel plate. This plate has a ladder-like structure that separates the molecules based on their electric charge.
Then, the scientists apply an electric current to the gel plate. This current helps the molecules move along the ladder and separate based on their electric charges. It's like sorting out the different types of candies in a jar based on their colors.
But that's not all! The gel plate also has different pH gradient zones. These zones are like different neighborhoods that the molecules pass through. As the molecules move along the ladder, they also move through these different pH zones, just like riding different trains to reach different destinations.
And eventually, each molecule reaches its own specific zone, where the pH is equal to its own isoelectric point. This is the point at which the molecule has zero net electric charge. It's like finding each candy's perfect spot in the candy jar based on their flavor!
So there you have it, kiddo! Isoelectric focusing is like a super cool method that scientists use to separate molecules based on their electric charges and find their specific spots on a gel plate based on the pH of different zones.
You know how we have different types of molecules in our body, right? And some of these molecules have an electric charge?
Isoelectric focusing is a method that scientists use to separate these molecules based on their electric charges. But how do they do this, you may ask?
Well, they start by placing the mixture of these molecules on a special gel plate. This plate has a ladder-like structure that separates the molecules based on their electric charge.
Then, the scientists apply an electric current to the gel plate. This current helps the molecules move along the ladder and separate based on their electric charges. It's like sorting out the different types of candies in a jar based on their colors.
But that's not all! The gel plate also has different pH gradient zones. These zones are like different neighborhoods that the molecules pass through. As the molecules move along the ladder, they also move through these different pH zones, just like riding different trains to reach different destinations.
And eventually, each molecule reaches its own specific zone, where the pH is equal to its own isoelectric point. This is the point at which the molecule has zero net electric charge. It's like finding each candy's perfect spot in the candy jar based on their flavor!
So there you have it, kiddo! Isoelectric focusing is like a super cool method that scientists use to separate molecules based on their electric charges and find their specific spots on a gel plate based on the pH of different zones.