ELI5: Explain Like I'm 5

Microbial DNA barcoding

Imagine that every living thing has a unique fingerprint that can help identify them, but instead of using ink, we use genetics! This is exactly what microbial DNA barcoding does.

Microbes are tiny living things that are too small to see with the naked eye. However, just like humans have unique DNA that distinguishes us from one another, the same is true for microbes. Scientists can use this DNA "fingerprint" to identify different types of microbes.

To get the DNA fingerprint, scientists start by collecting a sample of the microbe they want to study, for example, a sample of soil. They then use a special machine to extract the DNA from the microbes living in the soil. Once they have the DNA, they can use a process called PCR to amplify only certain parts of that DNA, which are unique to each microbe.

After the PCR process is complete, scientists can sequence the amplified DNA to find out which microbes are present in the sample. This sequence is like a code that can be used to identify specific microbes, just like when you input a secret code to unlock your phone.

This technique can be used to study microbes in all sorts of environments, from soil and water to the human gut. By identifying which microbes are present in a given environment, scientists can better understand how they interact with each other and their surroundings, and even develop new treatments for diseases caused by harmful microbes.

Overall, microbial DNA barcoding is a powerful tool that allows scientists to study and understand the world of tiny organisms, which is very important for our health and the environment.