ELI5: Explain Like I'm 5

Fast parallel proteolysis (FASTpp)

Have you ever seen a big puzzle with lots of little pieces? When you put all of the pieces together, you get a complete picture. Well, scientists have a puzzle too, but theirs is made up of tiny things called proteins. These proteins are like little machines that do different jobs to keep our bodies healthy.

But sometimes scientists need to take the proteins apart to see what they're made of and how they work. This is where fast parallel proteolysis (fastpp) comes in!

Fastpp is a way for scientists to break down lots of proteins at once so they can see how they're put together. It's kind of like having a bunch of different puzzles and taking all the pieces apart at the same time.

The way fastpp breaks down proteins is by using a special tool called a protease. Think of the protease as a little scissors that can cut the proteins into smaller pieces.

To use fastpp, scientists start by putting all of their proteins into a container. Then they add the protease to the container and let it do its work. The protease cuts the proteins into smaller pieces, just like the scissors cut paper.

But the cool thing about fastpp is that it doesn't just cut up one protein at a time. It can cut up lots of proteins all at once! That's why it's called "fast parallel proteolysis." Parallel means doing many things at the same time, like when you color with all of the crayons in the box at once.

Once the proteins are cut up, the scientists can look at the smaller pieces to see how they fit back together. Just like putting a puzzle back together, they can see how each piece fits with the others to make a complete picture of the protein.

And that's how fast parallel proteolysis works! It helps scientists take apart lots of proteins all at once so they can learn more about how they work.